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CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit (CQM006)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit - 1
  • CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit - 1
  • CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit - 2
  • CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit - 3
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All Size Price Qty
96T $ 495.00
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48T $ 396.00
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24T $ 150.00
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96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

The CellaQuantTM series of kits independently developed by Elabscience® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuantTM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level.

Sensitivity 9.38 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reactivity Mouse
Specificity This kit recognizes IL-2 in samples.No significant cross-reactivity or interference between IL-2 and analogues was observed.
Recovery 80%-120%
Sample Type Cell culture supernatant, Cell lysates or other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-2. Samples (or Standards &Quality Control Material) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse IL-2. You can calculate the concentration of Mouse IL-2 in the samples by comparing the OD of the samples to the standard curve.
Interleukin-2(IL-2), or T-cell growth factor(TCGF), is a 15–18 kDa glycosylated cytokine in the common gamma chain(γc)family, with a short half-life under 30 minutes. It is produced by activated CD4+and CD8+T cells, γδT cells, microglia, and hematopoietic stem cells.The IL-2 receptor consists of CD25(IL-2Rα), IL-2Rβ, and γc subunits. IL-2 binding to CD25 recruits IL-2Rβ and γc to form a signaling complex. IL-2Rβ is also used by IL-15, and γc is shared by multiple cytokines including IL-4, IL-7, and IL-15.IL-2 drives T cell activation and expansion in vitro. In vivo, it is essential for regulatory T cell(Treg)development and function, preventing autoimmunity, but can also promote inflammatory responses via Th2 cytokines and trafficking gene regulation. In CD8+T cells, IL-2 enhances effector differentiation and memory formation.
Gene ID 16183
Uniport ID P04351
Research Area Cancer , Immunology
CellaQuant™ Mouse IL-2 (Interleukin 2) ELISA Kit - procedures
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