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CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit (CQM002)

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CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - 1
  • CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - 1
  • CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - 2
  • CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - 3
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All Size Price Qty
96T $ 495.00
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48T $ 396.00
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24T $ 150.00
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96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

The CellaQuantTM series of kits independently developed by Elabscience® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuantTM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level.

Sensitivity 9.40 pg/mL
Detection Range 15.63-1000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reactivity Mouse
Specificity This kit recognizes TNF-α in samples.No significant cross-reactivity or interference between TNF-α and analogues was observed.
Recovery 80%-120%
Sample Type Cell culture supernatant, Cell lysates or other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Samples (or Standards &Quality Control Material) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.
Tumor Necrosis Factor-alpha (TNF-α), also known as cachectin or TNFSF1A, is the prototypic ligand of the Tumor Necrosis Factor superfamily. As a pleiotropic cytokine, TNF-α plays a central role in immunity, inflammation, and metabolic homeostasis. Its functions are primarily mediated by two receptors, TNFR1 and TNFR2, which activate signaling pathways such as NF-κB and MAPK to regulate a broad spectrum of cellular responses. In terms of immunity, TNF-α activates innate immune cells including macrophages and neutrophils, promotes the release of inflammatory cytokines such as IL-1 and IL-6, and modulates the activation and differentiation of T and B lymphocytes. In tissue homeostasis, TNF-α can induce apoptosis in target cells while also promoting cell survival via NF-κB signaling; under certain conditions, it also contributes to the induction of necroptosis. Metabolically, TNF-α inhibits lipoprotein lipase activity, leading to cachexia, and is involved in the development of insulin resistance. Dysregulation of TNF-α is closely associated with a variety of diseases, including autoimmune disorders such as rheumatoid arthritis and Crohn's disease, asthma, neuropathic pain, obesity and type 2 diabetes, septic shock, and the progression of various cancers. Human TNF-α is initially synthesized as a 26 kDa type II transmembrane protein, consisting of an intracellular domain of 35 amino acids, a transmembrane segment of 21 amino acids, and an extracellular domain of 177 amino acids. It is produced by a wide range of immune cells (such as macrophages and T cells), epithelial cells, endothelial cells, and certain tumor cells.
Gene ID 21926
Uniport ID P06804
Research Area Cancer , Stem Cells
CellaQuant™ Mouse TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit - procedures
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