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CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit (CQM007)

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CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit - 1
  • CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit - 1
  • CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit - 2
  • CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit - 3
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All Size Price Qty
96T $ 495.00
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48T $ 396.00
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24T $ 150.00
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96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary

The CellaQuantTM series of kits independently developed by Elabscience® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuantTM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level.

Sensitivity 18.75 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reactivity Mouse
Specificity This kit recognizes VEGF-A in samples.No significant cross-reactivity or interference between VEGF-A and analogues was observed.
Recovery 80%-120%
Sample Type Cell culture supernatant, Cell lysates or other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A. Samples (or Standards &Quality Control Material) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse VEGF-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse VEGF-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Mouse VEGF-A. You can calculate the concentration of Mouse VEGF-A in the samples by comparing the OD of the samples to the standard curve.
Vascular endothelial growth factor (VEGF-A/VEGF), originally termed vascular permeability factor (VPF), is a key regulator of angiogenesis and vasculogenesis during development and in adulthood. It belongs to the PDGF family, sharing the characteristic cystine knot motif and functioning as a disulfide-linked homodimer.VEGF is widely expressed in various tissues and cell types—including muscle, hepatocytes, osteoblasts, neutrophils, macrophages, and endothelial cells—and its production is induced by hypoxia and cytokines such as IL-1, IL-6, and TNF-α.During embryogenesis, VEGF drives endothelial cell proliferation, migration, and survival, thereby regulating vessel density and size. Postnatally, it supports endothelial integrity, wound healing, and female reproductive cycling. Pathologically, VEGF enhances vascular permeability and promotes tumor angiogenesis and metastasis. It also contributes to autoimmune conditions like rheumatoid arthritis and multiple sclerosis, making it a target for anti-angiogenic therapies.
Gene ID 22339
Uniport ID Q00731
Research Area Cancer , Cardiovascular , Metabolism , Developmental Biology , Signal Transduction
CellaQuant™ Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit - procedures
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