Name: CellaQuant™ TGF-β1 (Transforming Growth Factor Beta 1) ELISA Kit
Brand: Elabscience
SKU: CQ001
Price: 150.0 USD
Availability: InStock

Product Summary

"The CellaQuant^TM series of kits independently developed by Elabscience^® are specifically designed for the detection of cell samples, enabling accurate quantification of the expression levels of target proteins (such as cytokines) in cell supernatants and cell lysates. These products have been strictly optimized and thoroughly validated for typical cell samples, featuring high sensitivity, high specificity, and low background interference to ensure the accuracy and reliability of detection results. The CellaQuant^TM series is developed in strict accordance with clinical quality control standards. Some key parameters are traceable to to international standards, and they have excellent batch-to-batch consistency, providing a stable and reproducible analytical tool for protein expression research at the cellular level."

Sensitivity	0.089 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Universal
Specificity	This kit recognizes TGF-β1 in samples.No significant cross-reactivity or interference between TGF-β1 and analogues was observed.
Recovery	80%-120%
Sample Type	Cell culture supernatant, Cell lysates or other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Samples (or Standards &Quality Control Material) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Background

TGFB1 (TGF-β1) is a 44 kDa multifunctional cytokine regulating cell growth, differentiation, migration, immunity, and development. It exists as a latent proprotein, where the Latency-Associated Peptide (LAP) non-covalently binds TGF-β1, maintaining inactivity and storing it in the extracellular matrix. Activation is tightly controlled via interactions of LAP with milieu proteins (LTBP1, GARP, NRROS) and specific integrins (αvβ6/αvβ8), which induce conformational release of active TGF-β1.Active TGF-β1 signals through TGFBR1/TGFBR2 receptors, inducing SMAD2/3 phosphorylation and regulating gene expression. It modulates immune responses, directing T-cell differentiation toward Treg or Th17 lineages in a concentration-dependent manner. It is crucial for wound healing, bone remodeling, and organogenesis, but its dysregulation is linked to fibrosis, cancer progression, metastasis, atherosclerosis, and autoimmune diseases.