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DA(Dopamine) ELISA Kit (E-EL-0046)

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Citations ({{ citationsPage.numTotal }}) Manual MSDS
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DA(Dopamine) ELISA Kit - 1
  • DA(Dopamine) ELISA Kit - 1
  • DA(Dopamine) ELISA Kit - 2
  • DA(Dopamine) ELISA Kit - 3
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All Size Price Qty
96T $ 495.00
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48T $ 396.00
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24T $ 150.00
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96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 18.75 pg/mL
Detection Range 31.25-2000 pg/mL
Sample Volume 50 μL
Total Assay Time 2 h 30 min
Reactivity Universal
Specificity This kit recognizes Universal DA in samples.No significant cross-reactivity or interference between Universal DA and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal DA. During the reaction, Universal DA in the sample or standard competes with a fixed amount of Universal DA on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DA. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DA in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Dopamine (C6H3OH)2-CH2CH2NH2)is a catecholamine neurotransmitter in the brain. Its chemical name is 4-(2-aminoethyl)benzene-1,2-diol. Dopamine is a hormone released by the hypothalamus. Its main function is to inhibit the release of prolactin from the anterior lobe of the pituitary. It can be used as a sympathomimetic drug, producing effects such as increased heart rate and blood pressure.
Research Area Neuroscience
DA(Dopamine) ELISA Kit - procedures
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