Dihydrofolate Reductase (DHFR) Activity Colorimetric Assay Kit (E-BC-K816-M)
For research use only.
| Detection Principle |
Dihydrofolate Reductase (DHFR) is one of the key enzymes in nucleic acid metabolism and the main target enzyme in cancer drug therapy, and has been widely studied in recent years. The detection principle of this kit is as follows: DHFR catalyzes substrate reactions to consume NADPH. NADPH has a characteristic absorption peak at 340 nm. The activity of DHFR can be characterized by measuring the rate of decrease in absorbance at 340 nm. |
| Sample Type | Serum,Plasma,Animal and plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (optimum wavelength: 340 nm) |
| Research Area | Amino Acids And Proteins |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.02 U/L |
| Detection Range | 0.02-400 U/L |
| Precision | inter-assay CV: 5.0-7.2% | intra-assay CV: 2.5-3.6% |
| Sample Volume | 40 μL |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Rat serum | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Scindapsus aureus leaf tissue homogenate | 1 |
| 1×10^6 Hela cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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