E3(Estriol) ELISA Kit (E-EL-0156)
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For research use only.
Product Summary
| Sensitivity | 3.7 pg/mL |
| Detection Range | 7.81-500 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 2 h 30 min |
| Reactivity | Universal |
| Specificity | This kit recognizes Universal E3 in samples.No significant cross-reactivity or interference between Universal E3 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | serum, plasma |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal E3. During the reaction, Universal E3 in the sample or standard competes with a fixed amount of Universal E3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal E3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal E3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Background
Estriol is a hydroxylated metabolite of ESTRADIOL or ESTRONE that has a hydroxyl group at C3-beta, 16-alpha, and 17-beta position. Estriol is the main estrogen in the blood and urine of pregnant females. During pregnancy, large amount of estriol is produced by the placenta. Serum estriol determinations reflect the status of the fetus and are used to evaluate fetal distress and placental function.
| Research Area | Cell Biology , Signal Transduction , Developmental Biology |
| Cat.No. | Product Name | Clone No. |
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