F-TESTO(Free Testosterone) ELISA Kit (E-EL-0165)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal F-TESTO. During the reaction, Universal F-TESTO in the sample or standard competes with a fixed amount of Universal F-TESTO on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal F-TESTO. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal F-TESTO in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Testosterone is the principal androgen, or male sex hormone. One of the group of compounds known as anabolic steroids, testosterone is secreted by the testes but is also synthesized in small quantities in the ovaries, cortices of the adrenal glands, and placenta, usually from cholesterol. Testosterone is necessary in the fetus for the development of male external genitalia. Testosterone also stimulates protein synthesis and accounts for the greater muscular development of the male. For many years, synthetic steroids similar to testosterone have been used by athletes with the goal of improving performance, although recent medical research has shown that these drugs may have a wide range of harmful side effects.