Fluo-4 Calcium Fluorometric Assay Kit (E-BC-F100)
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For research use only.
| Detection Principle |
As an important metal ion in biological organism, calcium ion not only participates in the transmission of intracellular signals, but also plays an important role in many life activities of cells. Therefore, the detection of calcium ion concentration is of great significance in experimental research. This assay kit utilizes the cell-membrane-permeable calcium fluorescent probe Fluo-4 AM. The probe is non-fluorescent outside cells. Once inside cells, it is hydrolyzed by intracellular esterases to Fluo-4, which becomes trapped within the cytoplasm. Free Fluo-4 exhibits very weak fluorescence, but its fluorescence intensity increases significantly upon binding to Ca2+ . The probe shows maximum excitation at approximately 490 nm and maximum emission at 525 nm, and the fluorescence intensity is directly proportional to the intracellular calcium level. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=490 nm/525 nm), Fluorescence microscope (Ex/Em=542 nm/525 nm), Flow cytometry |
| Research Area | Inorganic Ions , Mitochondria Function |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Assay Time | 1 h 30 min |
| Cat.No. | Product Name | Clone No. |
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