GSH(Glutathione) ELISA Kit (E-EL-0026)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal GSH. During the reaction, Universal GSH in the sample or standard competes with a fixed amount of Universal GSH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal GSH. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal GSH in tested samples can be calculated by comparing the OD of the samples to the standard curve.

glutathione (r-glutamyl cysteingl +glycine (GSH)) is a tripeptide containing gamma-amide bonds and thiol groups, composed of glutamic acid, cysteine, and glycine, which is present in almost every cell of the body. Glutathione helps maintain normal immune system function and has antioxidant, integrative and detoxifying effects. Glutathione can not only be used as medicine, but also as the base material of functional food, which is widely used in functional food such as delaying aging, enhancing immunity and anti-tumor.