HoloTC(Holotranscobalamin) ELISA Kit (E-EL-0103)
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For research use only.
Product Summary
| Sensitivity | 4.69 fmol/mL |
| Detection Range | 7.81-500 fmol/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Universal |
| Specificity | This kit recognizes Universal HoloTC in samples.No significant cross-reactivity or interference between Universal HoloTC and analogues was observed |
| Recovery | 80%-120% |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal HoloTC. During the reaction, Universal HoloTC in the sample or standard competes with a fixed amount of Universal HoloTC on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal HoloTC. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal HoloTC in tested samples can be calculated by comparing the OD of the samples to the standard curve.
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