Human APLN(Apelin) ELISA Kit (E-EL-H0456)
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For research use only.
Product Summary
| Sensitivity | 37.5 pg/mL |
| Detection Range | 62.5-4000 pg/mL |
| Sample Volume | 50 μL |
| Total Assay Time | 2 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human APLN in samples.No significant cross-reactivity or interference between Human APLN and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, EDTA plasma, heparinized plasma, Saliva, breast milk, Urine, Semen, Stool and Cell culture supernatant |
| Detection Method | Colorimetric method, ELISA, Competitive |
| Assay Type | Competitive-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human APLN were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human APLN were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Human APLN was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human APLN and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human APLN. During the reaction, Human APLN in the sample or standard competes with a fixed amount of Human APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human APLN. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human APLN in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Background
Apelin is encoded by the APLN gene and is the endogenous ligand of the G-protein-coupled receptor APJ, previously an orphan receptor. Its 77-amino-acid precursor is cleaved into several active fragments, including apelin-36, apelin-17 and apelin-13, which act locally in autocrine and paracrine modes. Apelin is particularly abundant in cardiovascular and adipose tissue, where it behaves as an adipokine, and its expression is regulated by nutritional and metabolic factors, the multiple fragments adding complexity to the signaling system.
Activation of APJ by apelin promotes vasodilation, lowers blood pressure and enhances myocardial contractility, and contributes to fluid homeostasis by modulating vasopressin release and drinking behavior. Apelin also stimulates angiogenesis and glucose utilization. These cardiovascular and metabolic effects are mediated by APJ and act as compensatory protective signals under stress, influencing vascular tone, cardiac performance and energy metabolism.
Through the apelin-APJ axis the peptide regulates cardiovascular and metabolic physiology, and disturbed signaling is linked to heart failure, hypertension, obesity and insulin resistance. Quantitative determination of apelin is valuable in cardiometabolic research, supporting studies of cardiovascular disease and metabolic disorders and of receptor-targeted therapies for conditions such as heart failure with preserved ejection fraction and type 2 diabetes.
| Gene ID | 8862 |
| Uniport ID | Q9ULZ1 |
| Research Area | Cancer , Cardiovascular , Metabolism , Neuroscience , SignalTransduction |
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