Human BCAM (Basal Cell Adhesion Molecule) ELISA Kit (E-EL-H6305)

Name: Human BCAM (Basal Cell Adhesion Molecule) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6305
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Human BCAM (Basal Cell Adhesion Molecule) ELISA Kit - 1

Size	Price	Qty
96T	$ 495.00	- +
48T	$ 396.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	46.32 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes BCAM in samples.No significant cross-reactivity or interference between BCAM and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	2.221	2.297	2.259	2.184
2500	1.471	1.495	1.483	1.408
1250	0.838	0.850	0.844	0.769
625	0.510	0.496	0.503	0.428
312.5	0.292	0.298	0.295	0.220
156.25	0.183	0.169	0.176	0.101
78.13	0.131	0.125	0.128	0.053
0	0.078	0.072	0.075	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	275.600	566.880	2478.320	278.470	537.090	2268.980
Standard deviation	11.020	27.380	132.590	21.250	47.750	180.380
CV（%）	4.000	4.830	5.350	7.630	8.890	7.950

Recovery

The recovery of Human BCAM was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	89-100	94
Serum （n=8）	89-103	96
EDTA plasma （n=8）	88-102	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BCAM and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-109	84-99	93-108
1:2	Average	100	91	99
1:4	Range	90-106	84-97	94-106
1:4	Average	98	90	100
1:8	Range	97-113	85-98	90-101
1:8	Average	103	92	96

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	87.66-107.51
Sample 2（n=16）	93.75-95.04

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BCAM . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BCAM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BCAM , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BCAM . You can calculate the concentration of BCAM in the samples by comparing the OD of the samples to the standard curve.

Background

Human CD239 is a type I transmembrane glycoprotein that is encoded by B-CAM which belongs to the Ig gene superfamily. Full-length BCAM (CD239) is comprised of an extracellular region with two N-terminal V-type Ig-like domains followed by three C2-type Ig-like domains,a transmembrane region,and cytoplasmic domain of 59 amino acid residues. CD239 is expressed on erythrocytes in the peripheral blood and on the basal layer of vascular endothelial cells or some epithelial cells.