Human C4 (Complement Component 4) ELISA Kit (E-EL-H6309)

Name: Human C4 (Complement Component 4) ELISA Kit
Brand: Elabscience
SKU: E-EL-H6309
Price: 150.0 USD
Availability: InStock

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Human C4 (Complement Component 4) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes C4 in samples.No significant cross-reactivity or interference between C4 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	2.379	2.415	2.397	2.312
10	1.528	1.502	1.515	1.430
5	0.908	0.924	0.916	0.831
2.5	0.523	0.515	0.519	0.434
1.25	0.300	0.300	0.300	0.215
0.63	0.193	0.201	0.197	0.112
0.31	0.138	0.142	0.140	0.055
0	0.087	0.083	0.085	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	1.000	1.990	7.740	1.000	2.000	7.640
Standard deviation	0.060	0.130	0.310	0.060	0.120	0.530
CV（%）	5.690	6.370	4.010	6.210	6.210	6.990

Recovery

The recovery of Human C4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	93-108	100
Serum （n=8）	91-105	99
EDTA plasma （n=8）	90-102	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human C4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-112	95-111	96-114
1:2	Average	106	103	104
1:4	Range	94-109	92-107	92-106
1:4	Average	101	100	99
1:8	Range	99-114	95-109	98-111
1:8	Average	107	101	105

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	92.98-109.83
Sample 2（n=16）	94.63-113.79

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for C4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of C4 . You can calculate the concentration of C4 in the samples by comparing the OD of the samples to the standard curve.

Background

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.