Human CFD (Complement Factor D) ELISA Kit

Size	Price	Qty
96T	$ 609.00
48T	$ 487.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes CFD in samples.No significant cross-reactivity or interference between CFD and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFD . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFD , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CFD . You can calculate the concentration of CFD in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.

Uniport ID	P00746
Research Area	Immunology

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Human CFD (Complement Factor D) ELISA Kit (E-EL-H6278)

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