Human COL1α2(Collagen Type Ⅰ Alpha 2) ELISA Kit (E-EL-H0776)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human COL1α2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human COL1α2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human COL1α2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human COL1α2. You can calculate the concentration of Human COL1α2 in the samples by comparing the OD of the samples to the standard curve.

Collagen alpha-2(I) chain (COL1alpha2) is encoded by the COL1A2 gene and constitutes the pro-alpha2(I) subunit of type I collagen. Type I collagen is the most abundant structural protein in humans, assembled as a triple helix of two alpha1(I) chains and one alpha2(I) chain, and this supramolecular arrangement provides tissues such as bone, tendon and skin with resistance to tensile force. The alpha2(I) chain is synthesized principally by osteoblasts and fibroblasts and is essential for extracellular matrix integrity and skeletal development. After secretion, procollagen is converted to collagen by removal of the N- and C-propeptides, and the resulting fibrils are stabilized by lysyl oxidase-mediated lysine cross-links. Mature fibers turn over slowly but continuously, and proteolytic degradation releases characteristic collagen fragments into blood, so circulating fragment levels reflect ongoing matrix turnover. COL1A2 mutations cause osteogenesis imperfecta and certain Ehlers-Danlos syndromes. Measurement of type I collagen metabolites monitors bone turnover, fibrosis and wound healing, and is widely applied in osteoporosis and matrix-remodeling research. This ELISA kit enables quantitative determination of collagen alpha-2(I) chain in serum, plasma and other biological samples, and abnormal results are closely associated with the phenotype of connective tissue diseases, making the assay useful for evaluating the dynamic balance between collagen synthesis and degradation.