Human CRP(C-Reactive Protein) ELISA Kit (E-EL-H0043)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

C-reactive protein (CRP), also designated pentraxin 1, is a non-glycosylated member of the pentraxin family. It is synthesized mainly by hepatocytes and assembled as a cyclic pentamer of about 110-120 kDa. In humans CRP is a major acute-phase protein whose circulating concentration rises sharply during inflammation, where it acts as a sensor and activator of innate immunity. Transcription is driven mainly by interleukin-6 (IL-6), whereas in mice pentraxin 2 compensates, illustrating species differences. Through the ligand-binding face of its pentamer, CRP recognizes damaged self structures and microbial targets. It binds and opsonizes apoptotic cells and bacteria such as Streptococcus pneumoniae to enhance phagocytosis, and engages complement components including C1q, C4b-binding protein and factor H, promoting classical-pathway activation while limiting terminal lysis. Engagement of Fc gamma receptors links this humoral recognition to cellular effector functions. Because of its IL-6-driven dynamic response, CRP is the most widely used marker of inflammation and infection. Concentrations increase in bacterial infection, postoperative complications, active autoimmune disease and elevated cardiovascular risk, and serial measurement allows therapeutic response to be assessed. CRP therefore remains a first-line laboratory indicator of systemic inflammatory activity.