Human Irisin ELISA Kit (E-EL-H5735)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Irisin and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Irisin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Irisin. You can calculate the concentration of Human Irisin in the samples by comparing the OD of the samples to the standard curve.

Irisin is a secreted myokine released by proteolytic cleavage of the extracellular portion of the type I membrane protein fibronectin type III domain-containing protein 5 (FNDC5). The approximately 112-amino-acid fragment corresponds to the fibronectin type III-like domain and enters the circulation after removal of the amino-terminal signal peptide and shedding of the transmembrane segment; the sequence is highly conserved among mammals, indicating strong evolutionary constraint. Physical activity induces skeletal muscle expression of FNDC5 through peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1alpha), and the released myokine promotes browning of white adipose tissue by upregulating uncoupling protein 1 and mitochondrial content, thereby increasing thermogenesis and energy expenditure. Irisin also improves glucose tolerance, lipid handling and insulin sensitivity, and exerts effects on bone remodeling and neuroprotection in experimental models. Circulating irisin is reported to be lower in obesity, type 2 diabetes, metabolic syndrome and non-alcoholic fatty liver disease, and to rise acutely after exercise, although reported concentrations vary considerably depending on the assay used. Its role as a messenger in muscle-fat crosstalk makes it intensively studied. This ELISA kit enables quantitative determination of irisin in serum and plasma, supporting research on energy metabolism, adipose browning, exercise physiology and metabolic disease.