Human LZM(Lysozyme) ELISA Kit (E-EL-H1869)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human LZM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LZM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LZM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human LZM. You can calculate the concentration of Human LZM in the samples by comparing the OD of the samples to the standard curve.

Lysozyme (LZM), also called muramidase, is a small globular C-type lysozyme of about 14.7 kDa encoded by the LYZ gene. It hydrolyzes the beta-1,4-glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine in bacterial peptidoglycan and thereby lyses mainly Gram-positive bacteria. Produced by phagocytes, granulocytes and epithelial cells, it is secreted into tears, saliva, milk, plasma and urine and constitutes a first-line component of mucosal and systemic innate antimicrobial defense. As a muramidase, lysozyme weakens the peptidoglycan layer of susceptible bacteria and cooperates with secretory immunoglobulins, complement and antimicrobial peptides to potentiate host defense, while bacterial inhibitors such as Ivy and the peptidoglycan O-acetyltransferase counteract it. Its release reflects granulocyte turnover and monocyte-macrophage activation, and the enzyme also participates in digestion of bacterial debris, shaping of the commensal flora and modulation of mucosal inflammation. Elevated serum and urinary lysozyme accompany myeloid leukemia, renal tubular injury, sarcoidosis, tuberculosis and inflammatory bowel disease. Because lysozyme is freely filtered by the glomerulus and largely reabsorbed and catabolized by proximal tubular cells, increased urinary lysozyme is a sensitive indicator of proximal tubular dysfunction, and certain LYZ mutations cause hereditary systemic lysozyme amyloidosis. Measurement of lysozyme supports assessment of innate immunity and renal tubular integrity.