Human NEFL(Neurofilament, Light Polypeptide) ELISA Kit (E-EL-H6203)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human NEFL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NEFL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NEFL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human NEFL. You can calculate the concentration of Human NEFL in the samples by comparing the OD of the samples to the standard curve.

Human Clara cell 16 kDa protein (CC16), also called club cell secretory protein (CCSP), CC10, or secretoglobin family 1A member 1, is a small secreted protein of the secretoglobin family. It is produced predominantly by non-ciliated bronchiolar club cells of the distal airway and by other mucosae exposed to the external environment. The protein circulates as a non-covalent homodimer and is one of the most abundant proteins recovered from bronchoalveolar lavage fluid. CC16 binds phospholipids and inhibits phospholipase A2, exerting anti-inflammatory, immunomodulatory, and cytoprotective effects. It dampens inflammatory cell activation and chemotaxis and protects the respiratory epithelium from oxidative and proteolytic injury, thereby maintaining airway mucosal homeostasis. Because the protein diffuses readily across the alveolar-capillary barrier into blood, circulating concentrations reflect pulmonary epithelial integrity and barrier permeability, while secreted amounts also depend on club cell number and differentiation. Serum and urine CC16 serve as markers of pulmonary epithelial injury. Reduced circulating CC16 is associated with chronic obstructive pulmonary disease and asthma, whereas acute increases indicate toxic or infectious epithelial damage. This ELISA kit enables quantitative determination of human CC16 in serum, plasma, urine, bronchoalveolar lavage fluid, and cell culture supernatant, supporting occupational, environmental, and chronic lung disease research.