Human pMAPT /pTAU(phosphorylated microtubule-associated protein tau) ELISA Kit (E-EL-H5314)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human pMAPT/pTAU. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human pMAPT/pTAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human pMAPT/pTAU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human pMAPT/pTAU. You can calculate the concentration of Human pMAPT/pTAU in the samples by comparing the OD of the samples to the standard curve.

Phosphorylated tau (p-tau) denotes the abnormally hyperphosphorylated forms of the microtubule-associated protein tau encoded by MAPT. Native tau is a soluble phosphoprotein enriched in neurons, particularly within axons, where its microtubule-binding repeat domain stabilizes microtubule assembly and thereby maintains axonal transport and cytoskeletal organization; alternative splicing of the transcript generates isoforms containing three or four microtubule-binding repeats. In disease, imbalance between tau kinases and phosphatases drives hyperphosphorylation, causing tau to detach from microtubules and to self-assemble into paired helical filaments that accumulate as neurofibrillary tangles. Loss of normal function together with gain of toxicity disrupts axonal transport, synaptic integrity and neuronal viability, and pathological assemblies propagate between anatomically connected regions. Distinct residues differ in diagnostic specificity, with p-tau181, p-tau217 and p-tau231 among the most informative sites. Cerebrospinal fluid p-tau rises early in Alzheimer disease, and sensitive assays now allow such increases to be detected in blood. This ELISA kit enables quantitative determination of phosphorylated tau, and measurement together with amyloid-beta and total tau supports early diagnosis, differential diagnosis and monitoring of neurodegenerative disease, making p-tau an important indicator in neurological research and a valuable adjunct in the diagnosis of neurodegenerative disorders.