Human PTX 3/TSG-14(Pentraxin 3) ELISA Kit (E-EL-H6081)
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For research use only.
Product Summary
| Sensitivity | 4.12 pg/mL |
| Detection Range | 6.86-5000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes Human PTX3/TSG-14 in samples.No significant cross-reactivity or interference between Human PTX3/TSG-14 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTX3/TSG-14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTX3/TSG-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTX3/TSG-14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTX3/TSG-14. You can calculate the concentration of Human PTX3/TSG-14 in the samples by comparing the OD of the samples to the standard curve.
Background
| Gene ID | 5806 |
| Uniport ID | P26022 |
| Research Area | Cardiovascular , Immunology , Cancer , Cardiovascular |
| Cat.No. | Product Name | Clone No. |
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