Human RANκL(Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit (E-EL-H1387)

Name: Human RANκL(Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit
Brand: Elabscience
SKU: E-EL-H1387
Price: 150.0 USD
Availability: InStock

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Human RANκL(Receptor Activator of Nuclear Factor Kappa B Ligand) ELISA Kit - 1

Size	Price	Qty
96T	$ 700.00	- +
48T	$ 595.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

Sensitivity	0.1 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human RANκL in samples. No significant cross-reactivity or interference between Human RANκL and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	2.280	2.314	2.297	2.209
5	1.565	1.597	1.581	1.493
2.5	0.885	0.847	0.866	0.778
1.25	0.487	0.513	0.500	0.412
0.63	0.276	0.258	0.267	0.179
0.32	0.183	0.177	0.180	0.092
0.16	0.132	0.144	0.138	0.050
0	0.084	0.092	0.088	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.500	1.400	3.500	0.500	1.300	3.300
Standard deviation	0.030	0.070	0.160	0.040	0.050	0.140
CV（%）	6.000	5.000	4.570	8.000	3.850	4.240

Recovery

The recovery of Human RANκL was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	87-102	93
Serum （n=8）	92-106	98
EDTA plasma （n=8）	90-106	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human RANκL and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-97	85-96	85-97
1:2	Average	90	90	92
1:4	Range	93-105	88-100	97-114
1:4	Average	99	93	105
1:8	Range	95-109	87-98	96-113
1:8	Average	103	92	103

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	91.69-107.49
Sample 2（n=16）	89.71-103.53

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RANκL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RANκL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RANκL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human RANκL. You can calculate the concentration of Human RANκL in the samples by comparing the OD of the samples to the standard curve.

Background

RANκL is a cytokine that binds to TNFRSF11B/OPG and TNFRSF11A/RANK.Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.