Human TLR4(Toll-Like Receptor 4) ELISA Kit (E-EL-H5820)

Name: Human TLR4(Toll-Like Receptor 4) ELISA Kit
Brand: Elabscience
SKU: E-EL-H5820
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Human TLR4(Toll-Like Receptor 4) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	3.75 pg/mL
Detection Range	6.25-400 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human TLR4 in samples.No significant cross-reactivity or interference between Human TLR4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
400	2.310	2.398	2.354	2.281
200	1.687	1.689	1.688	1.615
100	0.974	1.016	0.995	0.922
50	0.538	0.540	0.539	0.466
25	0.308	0.318	0.313	0.240
12.5	0.182	0.172	0.177	0.104
6.25	0.125	0.127	0.126	0.053
0	0.077	0.069	0.073	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	21.350	56.350	137.360	21.530	54.180	145.470
Standard deviation	1.230	3.810	6.180	1.510	3.520	13.080
CV（%）	5.750	6.760	4.500	7.030	6.500	8.990

Recovery

The recovery of Human TLR4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	87-98	92
Serum （n=8）	100-110	103
EDTA plasma （n=8）	98-111	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human TLR4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-108	94-107	99-109
1:2	Average	100	101	105
1:4	Range	95-107	97-112	100-112
1:4	Average	103	105	105
1:8	Range	95-104	89-102	99-107
1:8	Average	100	95	103

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	88.91-111.59
Sample 2（n=16）	99.82-107.74

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TLR4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TLR4. You can calculate the concentration of Human TLR4 in the samples by comparing the OD of the samples to the standard curve.

Background