Human TM(Thrombomodulin) ELISA Kit (E-EL-H0166)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TM. You can calculate the concentration of Human TM in the samples by comparing the OD of the samples to the standard curve.

Thrombomodulin(TM),also known as CD141 and BDCA3,is a transmembrane protein expressed on vascular endothelial cells (EC),arterial smooth muscle cells,monocytes and macrophages.It binds to thrombin and enhances the activation of thrombin mediated anticoagulant protein C and antifibrinolytic TAFI/ carboxypeptidase B2.Coagulation regulatory proteins can also inhibit the ability of thrombin to activate several procoagulant proteins (such as fibrinogen,factor V,factor XIII,PAR-1).