Lactate Dehydrogenase (LDH) Activity Colorimetric Assay Kit (Lactate Substrate Method) (E-BC-K1302-M)
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For research use only.
| Detection Principle | Lactate Dehydrogenase (LDH) is widely present in animal tissues, with the highest content in the kidneys, followed by the myocardium and bone muscle. It is one of the important enzyme systems for anaerobic glycolysis and gluconeogenesis. LDH catalyzes the generation of NADH from substrates. The activity of LDH can be characterized by measuring the rate of increase in absorbance at 340 nm. |
| Sample Type | Animal tissue,Cell,Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (340 nm) |
| Research Area | Liver And Renal Function,Glycolysis And Lipid Metabolism,Energy Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 10 U/L |
| Detection Range | 10-1935 U/L |
| Precision | inter-assay CV: 2.0%-8.2% | intra-assay CV: 0.8%-2.2% |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1-2 |
| 10% Mouse liver tissue homogenate | 5-10 |
| 10% Mouse kidney tissue homogenate | 5-10 |
| 1×10^6 K562 Cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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