LTD4(Leukotriene D4) ELISA Kit (E-EL-0113)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal LTD4. During the reaction, Universal LTD4 in the sample or standard competes with a fixed amount of Universal LTD4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTD4. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal LTD4 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

LTD4 is associated with the pathogenesis of several inflammatory disorders, such as asthma and inflammatory boweldisease . A general link between chronic inflammationand increased risk of developing cancer is possible. There are that exposure to LTD4 increases survival and proliferation inintestinal epithelial cells . the CysLT1 regulator is up-regulated in coloncancer tissue, and that LTD4 signalling facilitates the survivalof cancer cells . LTD4 could reduce apoptosis in non-transformed epithelialcells