Micro-Sample

Mini Sample Human BDNF ( Brain Derived Neurotrophic Factor ) ELISA Kit (E-MSEL-H0004)

Name: Mini Sample Human BDNF ( Brain Derived Neurotrophic Factor ) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0004
Price: 150.0 USD
Availability: InStock

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Mini Sample Human BDNF ( Brain Derived Neurotrophic Factor ) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	56.25 pg/mL
Detection Range	93.75-6000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes BDNF in samples.No significant cross-reactivity or interference between BDNF and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
6000	2.288	2.320	2.304	2.213
3000	1.447	1.475	1.461	1.370
1500	0.825	0.827	0.826	0.735
750	0.527	0.543	0.535	0.444
375	0.302	0.304	0.303	0.212
187.5	0.184	0.192	0.188	0.097
93.75	0.139	0.141	0.140	0.049
0	0.091	0.091	0.091	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	318.150	951.040	2582.120	336.260	1010.620	2638.700
Standard deviation	14.540	40.700	149.500	27.370	77.410	187.080
CV（%）	4.570	4.280	5.790	8.140	7.660	7.090

Recovery

The recovery of Human BDNF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-103	98
EDTA plasma （n=8）	94-109	100
Cell culture media （n=8）	89-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BDNF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-109	87-101	91-105
1:2	Average	101	93	97
1:4	Range	94-110	83-97	90-105
1:4	Average	102	90	97
1:8	Range	94-110	87-98	95-107
1:8	Average	102	92	100

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	93.92-114.83
Sample 2（n=16）	95.22-100.15

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BDNF . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BDNF , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BDNF . You can calculate the concentration of BDNF in the samples by comparing the OD of the samples to the standard curve.

Background

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammals examined. All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins. These proneurotrophins have distinct biological activity different from their mature counterparts. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.