Mini Sample Human BDNF ( Brain Derived Neurotrophic Factor ) ELISA Kit (E-MSEL-H0004)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BDNF . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BDNF , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BDNF . You can calculate the concentration of BDNF in the samples by comparing the OD of the samples to the standard curve.

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins) that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).  BDNF shares approximately 55% amino acid identity with other family members.  The amino acid sequence of mature BDNF is identical in all mammals examined.  All neurotrophins are noncovalently linked homodimers and initially produced as proneurotrophins.  These proneurotrophins have distinct biological activity different from their mature counterparts.  Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development.  BDNF participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology.  BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS.