Micro-Sample

Mini Sample Human CASP3 (Caspase 3) ELISA Kit (E-MSEL-H0021)

Name: Mini Sample Human CASP3 (Caspase 3) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0021
Price: 150.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Resources

Mini Sample Human CASP3 (Caspase 3) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes CASP3 in samples.No significant cross-reactivity or interference between CASP3 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	2.277	2.251	2.264	2.184
5	1.539	1.571	1.555	1.475
2.5	0.998	1.016	1.007	0.927
1.25	0.520	0.512	0.516	0.436
0.63	0.285	0.287	0.286	0.206
0.31	0.184	0.172	0.178	0.098
0.16	0.119	0.120	0.120	0.040
0	0.083	0.077	0.080	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.530	1.050	4.270	0.580	1.020	4.430
Standard deviation	0.040	0.040	0.190	0.040	0.080	0.400
CV（%）	6.780	4.020	4.560	7.350	7.820	8.920

Recovery

The recovery of Human CASP3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-107	100
EDTA plasma （n=8）	86-100	92
Cell culture media （n=8）	92-105	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CASP3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-113	96-112	88-104
1:2	Average	103	103	96
1:4	Range	99-111	92-103	90-103
1:4	Average	106	98	97
1:8	Range	97-111	96-109	86-100
1:8	Average	104	101	93

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	88.60-111.01
Sample 2（n=16）	100.85-104.89

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CASP3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CASP3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CASP3 . You can calculate the concentration of CASP3 in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease ( caspase ) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits , large and small , that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 , 7 and 9 , and the protein itself is processed by caspases 8 , 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein , which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.