Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes CASP3 in samples.No significant cross-reactivity or interference between CASP3 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CASP3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CASP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CASP3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CASP3 . You can calculate the concentration of CASP3 in the samples by comparing the OD of the samples to the standard curve.

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease ( caspase ) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits , large and small , that dimerize to form the active enzyme. This protein cleaves and activates caspases 6 , 7 and 9 , and the protein itself is processed by caspases 8 , 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein , which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

Gene Alias	CASP3
Gene ID	836
Uniport ID	P42574
Protein Alias	CASP3
Research Area	Cancer , Cell Biology , Metabolism , Neuroscience

Mini Sample Human CASP3 (Caspase 3) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Human CASP3 (Caspase 3) ELISA Kit (E-MSEL-H0021)

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