Micro-Sample
Mini Sample Human CXCL1 (Growth Regulated Oncogene Alpha) ELISA Kit (E-MSEL-H0037)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 46.88 pg/mL |
| Detection Range | 78.13-5000 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes CXCL1 in samples.No significant cross-reactivity or interference between CXCL1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Human CXCL1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human CXCL1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CXCL1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CXCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CXCL1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 37 nm. The OD value is proportional to the concentration of CXCL1 . You can calculate the concentration of CXCL1 in the samples by comparing the OD of the samples to the standard curve.
Background
CXCL1/ GROα, also known as KC in mice and cinc1 in rats, is a member of the CXC chemokine family. Human CXCL1/ GROα has a total length of 107 amino acids (aa) with an estimated molecular weight of 11 kDa. The sequence homology of mouse and rat homologues to human protein was 57% and 59%, respectively. Genome-wide analysis showed that the protein encoded by the mouse Cxcl3 gene was homologous to the human CXCL1/ GROα protein. Human CXCL1/ GROα can be cleaved into three isoforms: CXCL1/ GROα (aa 4-73), CXCL1/ GROα (aa 5-73) and CXCL1/ GROα (aa 6-73), all of which are more active than full-length proteins. CXCL1/ GROα is secreted by mast cells and macrophages, so it acts as a chemical attractant of neutrophils; GRO-α plays an important role in inflammation and immune response, enhances immune defense by attracting and activating immune cells, is up-regulated in the tumor microenvironment, and is closely related to tumor growth, angiogenesis and metastasis. It can promote tumor progression by activating tumor-associated fibroblasts and endothelial cells.
| Gene Alias | CXCL1 |
| Gene ID | 2919 |
| Uniport ID | P09341 |
| Protein Alias | CXCL1 |
| Research Area | Immunology , InnateImmunity , Chemokines , SignalTransduction , SignalingPathway , Neuroscience |
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