Micro-Sample
Mini Sample Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit (E-MSEL-H0031)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 9.38 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes ESM1 in samples.No significant cross-reactivity or interference between ESM1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Performance
| Typical data |
The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only.
Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.
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| Precision |
Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.
Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.
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| Recovery |
The recovery of Human ESM1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.
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| Linearity |
Linearity of the assay was evaluated by spiking samples with high concentrations of Human ESM1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range.
The measured values were then compared to the expected concentrations to assess the linearity of response.
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| Stability |
Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.
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Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ESM1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ESM1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 31 nm. The OD value is proportional to the concentration of ESM1 . You can calculate the concentration of ESM1 in the samples by comparing the OD of the samples to the standard curve.
Background
Endothelial cells are multifunctional cells that cover the inner surface of blood vessels and regulate a variety of important physiological and pathological reactions. Endothelial cells are dynamic participants in cell and organ function, rather than a passive barrier. Through the expression of surface proteins and the secretion of soluble mediators, endothelial cells control vascular tone and permeability, regulate coagulation and thrombosis, and direct the recruitment and homing of white blood cells to specific areas of inflammation. This endothelial cell dependent control of white blood cell roll, adhesion, and transendothelial migration involves several known endothelial cell surface molecules, including E- and P-selectin, ICAMs, and VCAM-1, which are differentially regulated by pro-inflammatory cytokines such as TNF· and IFNA.
| Gene Alias | ESM1 |
| Gene ID | 11082 |
| Uniport ID | Q9NQ30 |
| Protein Alias | ESM1 |
| Research Area | TagsandCellMarkers |
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