Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes ESM1 in samples.No significant cross-reactivity or interference between ESM1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ESM1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ESM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ESM1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 31 nm. The OD value is proportional to the concentration of ESM1 . You can calculate the concentration of ESM1 in the samples by comparing the OD of the samples to the standard curve.

Background

Endothelial cells are multifunctional cells that cover the inner surface of blood vessels and regulate a variety of important physiological and pathological reactions. Endothelial cells are dynamic participants in cell and organ function, rather than a passive barrier. Through the expression of surface proteins and the secretion of soluble mediators, endothelial cells control vascular tone and permeability, regulate coagulation and thrombosis, and direct the recruitment and homing of white blood cells to specific areas of inflammation. This endothelial cell dependent control of white blood cell roll, adhesion, and transendothelial migration involves several known endothelial cell surface molecules, including E- and P-selectin, ICAMs, and VCAM-1, which are differentially regulated by pro-inflammatory cytokines such as TNF· and IFNA.

Gene Alias	ESM1
Gene ID	11082
Uniport ID	Q9NQ30
Protein Alias	ESM1
Research Area	Tags And Cell Markers

Mini Sample Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Human ESM1 (Endothelial Cell Specific Molecule 1) ELISA Kit (E-MSEL-H0031)

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