Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	4.69 ng/mL
Detection Range	7.81-500 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Human
Specificity	This kit recognizes F9 in samples.No significant cross-reactivity or interference between F9 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to F9 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for F9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain F9 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 34 nm. The OD value is proportional to the concentration of F9 . You can calculate the concentration of F9 in the samples by comparing the OD of the samples to the standard curve.

Background

Human coagulation factor IX is is a vitamin K-dependent plasma protein, composed of 416 amino acid residues that plays a crucial role in the intrinsic pathway of blood coagulation by converting factor X into its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa. It is primarily synthesized in the liver and subsequently secreted into the plasma. During the intrinsic pathway of coagulation, activation of human coagulation factor IX releases a peptide segment, resulting in the formation of two polypeptide chains linked by disulfide bonds. The region that interacts with phospholipids resides in the light chain, while the catalytic active site of the enzyme is located in the heavy chain. Activated coagulation factor IX (IXa), in conjunction with activated coagulation factor VIII, activates coagulation factor X, facilitating the conversion of prothrombin to thrombin and thereby ensuring normal progression of the clotting process.Deficiencies in F9 are responsible for recessive X-linked hemophilia B (HEMB) , commonly referred to as Christmas disease. Notably, mutations at position 43 (Oxford-3, San Dimas) and position 46 (Cambridge) hinder propeptide cleavage; a mutation at position 93 (Alabama) likely disrupts binding to cell membranes; while mutations at positions 191 (Chapel-Hill) or 226 (Nagoya or Hilo) impede activation peptide cleavage.Additionally, defects in F9 can lead to thrombophilia associated with factor IX deficiency (THR-FIX) , which is characterized by an increased tendency toward thrombosis.

Gene Alias	F9
Gene ID	2158
Uniport ID	P00740
Protein Alias	F9
Research Area	Cell Biology , Signal Transduction , Stem Cells

Mini Sample Human F9 (Coagulation factor IX) ELISA Kit - procedures

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Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Mini Sample Human F9 (Coagulation factor IX) ELISA Kit (E-MSEL-H0034)

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