Mini Sample Human HCG ( Chorionic Gonadotropin ) ELISA Kit (E-MSEL-H0006)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HCG . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HCG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HCG , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of HCG . You can calculate the concentration of HCG in the samples by comparing the OD of the samples to the standard curve.

Human Chorionic Gonadotropin (hCG) is a hormone produced by the placenta and its main role is to promote fetal growth and maintain pregnancy. In addition to its wide use in pregnancy testing, the level of hCG can also be used as a biomarker for some diseases, such as some tumors. In addition, hCG can also be used as an indicator of some drugs to judge the efficacy of drug treatment.