Mini Sample Human IL-3 (Interleukin-3) ELISA Kit (E-MSEL-H0028)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 28 nm. The OD value is proportional to the concentration of IL-3 . You can calculate the concentration of IL-3 in the samples by comparing the OD of the samples to the standard curve.

Interleukin-3 (lL-3) is a hemopoietic growth factor involved in the survival,proliferation,and differentiation of multipotent hemopoietic cells. In addition to granulocyte-macrophage colony-stimulating factor (GMCSF) and IL-5,IL-3 is the most potent growth factor for basophils. On mature basophils,these three cytokines act through specific receptors mediating adhesion,migration,and releasability. Mast cells expresses high amounts of IL-3. In the absence of antigen,rapid release of large amount of IL-3 in an autocrine dependent manner is responsible for mast cell survival. IL-3 plays a vital role in stimulating basophils and mast cell responses to parasite infections. IL-3 has also been implicated in the pathogenesis of several chronic inflammatory diseases,including asthma,atherosclerosis,and neurodegenerative disorders,such as multiple sclerosis.  IL-3 stimulates colony formation of megakaryocytes,neutrophils,and macrophages from bone marrow cultures. IL-3 is expressed in the major embryonic vessels and regulates the survival and proliferation of hematopoietic stem cells in the early stages of embryonic development.