Mini Sample Human IL-6 (Interleukin 6) ELISA Kit (E-MSEL-H0025)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 7.5 pg/mL |
| Detection Range | 12.5-800 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes IL-6 in samples.No significant cross-reactivity or interference between IL-6 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-6 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-6 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 25 nm. The OD value is proportional to the concentration of IL-6 . You can calculate the concentration of IL-6 in the samples by comparing the OD of the samples to the standard curve.
Background
IL-6 (interleukin-6) is a cytokine produced by a variety of cells that plays an important role in the immune system and inflammatory response. The biological meaning of IL-6 plays an important role in immune regulation, infection resistance, hematopoietic regulation and so on. In addition, it is also involved in the occurrence and development of many diseases, such as tumors, autoimmune diseases, cardiovascular diseases and so on.
| Gene Alias | IL6 |
| Gene ID | 3569 |
| Uniport ID | P05231 |
| Protein Alias | IL6 |
| Research Area | Immunology |
| Cat.No. | Product Name | Clone No. |
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