Mini Sample Human KIM-1 (Kidney Injury Molecule 1) ELISA Kit (E-MSEL-H0033)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 0.19 ng/mL |
| Detection Range | 0.31-20 ng/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes KIM-1 in samples.No significant cross-reactivity or interference between KIM-1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to KIM-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for KIM-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain KIM-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 33 nm. The OD value is proportional to the concentration of KIM-1 . You can calculate the concentration of KIM-1 in the samples by comparing the OD of the samples to the standard curve.
Background
Kim-1, Kidney Injury molecule-1, belongs to the surface Molecule of renal tubular epithelial cells and is an important marker of acute kidney injury. The expression of Kim-1 is low under normal conditions, but it is significantly up-regulated after renal tubular epithelial cells are injured. Therefore, Kim-1 can be used as a sensitive index for early diagnosis of acute kidney injury, which is of great significance for the diagnosis and monitoring of kidney diseases.
| Gene Alias | HAVCR1 |
| Gene ID | 26762 |
| Uniport ID | Q96D42 |
| Protein Alias | HAVCR1 |
| Research Area | Immunology |
| Cat.No. | Product Name | Clone No. |
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