Micro-Sample

Mini Sample Human MCP-1 ( Monocyte Chemotactic Protein 1 ) ELISA Kit (E-MSEL-H0013)

Name: Mini Sample Human MCP-1 ( Monocyte Chemotactic Protein 1 ) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-H0013
Price: 150.0 USD
Availability: InStock

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Mini Sample Human MCP-1 ( Monocyte Chemotactic Protein 1 ) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes MCP-1 in samples.No significant cross-reactivity or interference between MCP-1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.247	2.191	2.219	2.125
500	1.414	1.372	1.393	1.299
250	0.846	0.832	0.839	0.745
125	0.450	0.478	0.464	0.370
62.5	0.288	0.274	0.281	0.187
31.25	0.182	0.188	0.185	0.091
15.63	0.143	0.145	0.144	0.050
0	0.093	0.095	0.094	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	45.510	154.440	491.510	42.490	148.400	452.800
Standard deviation	1.840	10.040	32.830	3.210	11.120	32.510
CV（%）	4.040	6.500	6.680	7.550	7.490	7.180

Recovery

The recovery of Human MCP-1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-104	97
EDTA plasma （n=8）	84-98	91
Cell culture media （n=8）	91-102	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MCP-1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-112	98-112	95-112
1:2	Average	102	105	103
1:4	Range	92-107	99-113	92-105
1:4	Average	97	105	98
1:8	Range	89-106	100-114	95-110
1:8	Average	97	106	101

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	88.21-100.52
Sample 2（n=16）	91.11-91.87

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to MCP-1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MCP-1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of MCP-1 . You can calculate the concentration of MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Background

MCP-1, also known as CCL2, is a member of the CC chemokine family. It is widely expressed in endothelial cells, smooth muscle cells and monocytes in response to several atherogenic stimulants such as CD40 ligand, platelet derived growth factor (PDGF), interleukin-1β (IL-1β) and oxidized low density lipoprotein.