Mini Sample Human TFR1 (Transferrin Receptor 1) ELISA Kit (E-MSEL-H0024)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 75.01 pg/mL |
| Detection Range | 125-8000 pg/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Human |
| Specificity | This kit recognizes TFR1 in samples.No significant cross-reactivity or interference between TFR1 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TFR1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TFR1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TFR1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TFR1 . You can calculate the concentration of TFR1 in the samples by comparing the OD of the samples to the standard curve.
Background
sTfRs are blood proteins with increased expression in iron deficiency. It is the receptor for transferrin that binds to and carries iron to tissues and cells throughout the body. The measurement of sTfR levels can help to evaluate iron deficiency and diagnose iron-deficiency anemia. To increase sensitivity and specificity, iron status, including ferritin, TIBC, serum iron, and other relative tests are recommended to be performed along with the sTfR test
| Gene Alias | TFRC |
| Gene ID | 7037 |
| Uniport ID | P02786 |
| Protein Alias | TFRC |
| Research Area | Cardiovascular , Signal Transduction |
| Cat.No. | Product Name | Clone No. |
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