Mini Sample Mouse ANGPTL3 (Angiopoietin Like Protein 3) ELISA Kit (E-MSEL-M0110)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ANGPTL3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ANGPTL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ANGPTL3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ANGPTL3 . You can calculate the concentration of ANGPTL3 in the samples by comparing the OD of the samples to the standard curve.

Acts in part as a hepatokine that is involved in regulation of lipid and glucose metabolism.Proposed to play a role in the trafficking of energy substrates to either storage or oxidative tissues in response to food intake.Has a stimulatory effect on plasma triglycerides (TG), which is achieved by suppressing plasma TG clearance via inhibition of LPL activity;Can inhibit endothelial lipase, causing increased plasma levels of high density lipoprotein (HDL) cholesterol and phospholipids; the cleaved N-terminal domain is more efficient than the uncleaved proprotein.Can bind to adipocytes to activate lipolysis, releasing free fatty acids and glycerol.