Mini Sample Mouse CD40 (Cluster of Differentiation 40) ELISA Kit (E-MSEL-M0122)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 0.09 ng/mL |
| Detection Range | 0.16-10 ng/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Mouse |
| Specificity | This kit recognizes CD40 in samples.No significant cross-reactivity or interference between CD40 and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CD40 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CD40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CD40 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 47 nm. The OD value is proportional to the concentration of CD40 . You can calculate the concentration of CD40 in the samples by comparing the OD of the samples to the standard curve.
Background
CD40 protein receptor for TNFSF5/CD40LG.Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
| Gene Alias | Cd40 |
| Gene ID | 21939 |
| Uniport ID | P27512 |
| Protein Alias | Cd40 |
| Research Area | Cell Biology , Immunology |
| Cat.No. | Product Name | Clone No. |
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