Micro-Sample

Mini Sample Mouse CFH (Complement Factor H) ELISA Kit (E-MSEL-M0115)

Name: Mini Sample Mouse CFH (Complement Factor H) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0115
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse CFH (Complement Factor H) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes CFH in samples.No significant cross-reactivity or interference between CFH and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	2.383	2.371	2.377	2.305
50	1.420	1.464	1.442	1.370
25	0.842	0.854	0.848	0.776
12.5	0.470	0.474	0.472	0.400
6.25	0.279	0.261	0.270	0.198
3.13	0.166	0.174	0.170	0.098
1.56	0.114	0.120	0.117	0.045
0	0.073	0.071	0.072	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	5.550	10.470	40.010	5.110	10.920	40.590
Standard deviation	0.280	0.540	2.400	0.390	0.750	2.990
CV（%）	5.120	5.130	6.010	7.690	6.870	7.360

Recovery

The recovery of Mouse CFH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-97	92
EDTA plasma （n=8）	94-110	102
Cell culture media （n=8）	87-100	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CFH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	90-105	90-105
1:2	Average	97	96	96
1:4	Range	90-102	88-101	91-108
1:4	Average	96	95	99
1:8	Range	92-106	87-99	88-99
1:8	Average	99	93	94

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	96.75-99.90
Sample 2（n=16）	92.40-109.65

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFH . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFH , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 40 nm. The OD value is proportional to the concentration of CFH . You can calculate the concentration of CFH in the samples by comparing the OD of the samples to the standard curve.

Background

Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.