Mini Sample Mouse CFH (Complement Factor H) ELISA Kit (E-MSEL-M0115)
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For research use only.
Product Summary
The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.
| Sensitivity | 0.94 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Sample Volume | 25 μL |
| Manual Operation Time | 1 h |
| Total Assay Time | 3 h 30 min |
| Reactivity | Mouse |
| Specificity | This kit recognizes CFH in samples.No significant cross-reactivity or interference between CFH and analogues was observed. |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T / 48T / 24T / 96T*5 / 96T*10 |
| Storage | 2-8℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CFH . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CFH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CFH , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 40 nm. The OD value is proportional to the concentration of CFH . You can calculate the concentration of CFH in the samples by comparing the OD of the samples to the standard curve.
Background
Factor H functions as a cofactor in the inactivation of C3b by factor I and also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b)NBB complex (C5 convertase) in the alternative complement pathway.
| Gene Alias | Cfh |
| Gene ID | 12628 |
| Uniport ID | P06909 |
| Protein Alias | Cfh |
| Research Area | Immunology |
| Cat.No. | Product Name | Clone No. |
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