Micro-Sample

Mini Sample Mouse CHI3L1 (Chitinase 3-like 1) ELISA Kit (E-MSEL-M0109)

Name: Mini Sample Mouse CHI3L1 (Chitinase 3-like 1) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0109
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse CHI3L1 (Chitinase 3-like 1) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	37.51 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes CHI3L1 in samples.No significant cross-reactivity or interference between CHI3L1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	2.097	2.157	2.127	2.048
2000	1.383	1.331	1.357	1.278
1000	0.855	0.825	0.840	0.761
500	0.444	0.472	0.458	0.379
250	0.280	0.280	0.280	0.201
125	0.165	0.167	0.166	0.087
62.5	0.118	0.119	0.119	0.040
0	0.082	0.076	0.079	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	200.710	341.950	1573.370	215.220	361.100	1540.470
Standard deviation	12.120	15.800	90.780	16.140	30.950	94.280
CV（%）	6.040	4.620	5.770	7.500	8.570	6.120

Recovery

The recovery of Mouse CHI3L1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-97	92
EDTA plasma （n=8）	89-102	96
Cell culture media （n=8）	86-102	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse CHI3L1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-99	92-106	84-95
1:2	Average	90	97	90
1:4	Range	83-95	91-101	84-97
1:4	Average	90	96	90
1:8	Range	88-99	88-98	83-95
1:8	Average	93	93	89

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	88.18-99.17
Sample 2（n=16）	85.61-91.56

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CHI3L1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CHI3L1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CHI3L1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of CHI3L1 . You can calculate the concentration of CHI3L1 in the samples by comparing the OD of the samples to the standard curve.

Background

Chitinases catalyze the hydrolysis of chitin; which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages; chondrocytes; neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling.