Micro-Sample

Mini Sample Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit (E-MSEL-M0126)

Name: Mini Sample Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0126
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse DKK3 (Dickkopf Related Protein 3) ELISA Kit - 1

Size	Price	Qty
96T	$ 625.00	- +
48T	$ 500.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse DKK3 in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	2.349	2.271	2.310	2.223
20	1.613	1.655	1.634	1.547
10	0.923	0.903	0.913	0.826
5	0.465	0.489	0.477	0.390
2.5	0.304	0.292	0.298	0.211
1.25	0.183	0.195	0.189	0.102
0.63	0.139	0.141	0.140	0.053
0	0.091	0.083	0.087	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.010	5.650	14.910	2.040	5.360	15.850
Standard deviation	0.090	0.290	0.670	0.180	0.460	1.160
CV（%）	4.290	5.050	4.520	8.800	8.500	7.290

Recovery

The recovery of Mouse DKK3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	97
EDTA plasma （n=8）	86-100	93
Cell culture media （n=8）	95-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse DKK3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-109	90-102	86-100
1:2	Average	101	96	92
1:4	Range	95-108	90-100	86-98
1:4	Average	100	95	92
1:8	Range	96-111	87-102	83-95
1:8	Average	103	94	89

For the recovery experiment, the samples were spiked with standard solutions of a specific concentration and then serially diluted to 2-fold, 4-fold, 8-fold, and 16-fold dilutions, respectively. The average recovery rate was subsequently calculated.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse DKK3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse DKK3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse DKK3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 12 nm. The OD value is proportional to the concentration of Mouse DKK3. You can calculate the concentration of Mouse DKK3 in the samples by comparing the OD of the samples to the standard curve.

Background

Dkk3 protein antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.