Mini Sample Mouse GRN (Granulin) ELISA Kit (E-MSEL-M0112)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GRN . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GRN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GRN , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of GRN . You can calculate the concentration of GRN in the samples by comparing the OD of the samples to the standard curve.

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation.Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes.Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB.In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures.Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases .Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity .