Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes PAI1 in samples.No significant cross-reactivity or interference between PAI1 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PAI1 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PAI1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PAI1 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of PAI1 . You can calculate the concentration of PAI1 in the samples by comparing the OD of the samples to the standard curve.

Background

Serpin E1 is a member of the serine proteinase inhibitors (serpin) superfamily, which is divided into two groups: inhibitory and non- inhibitory serpins. Serpin E1 is the most important physiological inhibitor of tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA), and therefore is a key regulator of fibrinolysis via inhibition of the activation of the broad-spectrum serine protease plasmin. Serpin E1 forms a covalent bond with both t-PA and u-PA (1:1 molar ratio). It is known that several receptors (uPAR, LRP-1A, megalin, VLDLR, and LRP-1B) bind and mediate endocytosis of plasminogen activator-Serpin E1 complexes.

Gene Alias	Serpine1
Gene ID	18787
Uniport ID	P22777
Protein Alias	Serpine1
Research Area	Cardiovascular

Mini Sample Mouse PAI1 ( Plasminogen Activator Inhibitor 1 ) ELISA Kit - procedures

Other Clones

{{antibodyDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Other Formats

{{formatDetailsPage.numTotal}} Results

All

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

IF：{{item.impact}}

Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}
Reactivity：{{item.species}}

Sample Type：{{item.organization}}

Previous
{{ page }}
Next

Species

{{relatedElisaPage.numTotal}} Results

All

Human

Mouse

Rat

({{item.photos_.length}})

({{item.images_.length}})
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS

Cat.No.：{{item.cat}}

{{index}} {{goods_show_value}}

Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Previous
{{ page }}
Next

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

All categories

Mini Sample Mouse PAI1 ( Plasminogen Activator Inhibitor 1 ) ELISA Kit (E-MSEL-M0098)

Other Clones

Other Formats

Species