Mini Sample Mouse SP-D (Pulmonary surfactant-associated protein D) ELISA Kit

Size	Price	Qty
96T	$ 510.00
48T	$ 408.00
24T	$ 150.00
96T*5	Inquire	/
96T*10	Inquire	/

Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reacitivity	Mouse
Specificity	This kit recognizes SP-D in samples.No significant cross-reactivity or interference between SP-D and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SP-D . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for SP-D and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SP-D , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SP-D . You can calculate the concentration of SP-D in the samples by comparing the OD of the samples to the standard curve.

Background

Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Gene Alias	Sftpd
Gene ID	20390
Uniport ID	P50404
Protein Alias	Sftpd
Research Area	Immunology

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Mini Sample Mouse SP-D (Pulmonary surfactant-associated protein D) ELISA Kit (E-MSEL-M0113)

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