Micro-Sample

Mini Sample Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit (E-MSEL-M0127)

Name: Mini Sample Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-M0127
Price: 150.0 USD
Availability: InStock

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Mini Sample Mouse TFPI (Tissue Factor Pathway Inhibitor) ELISA Kit - 1

Size	Price	Qty
96T	$ 625.00	- +
48T	$ 500.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	25 μL
Total Assay Time	3 h 30 min
Reactivity	Mouse
Specificity	This kit recognizes Mouse TFPI in samples.No significant cross-reactivity or interference between and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T*5
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	2.346	2.402	2.374	2.298
1000	1.661	1.645	1.653	1.577
500	1.041	1.015	1.028	0.952
250	0.533	0.535	0.534	0.458
125	0.307	0.299	0.303	0.227
62.5	0.173	0.181	0.177	0.101
31.25	0.134	0.124	0.129	0.053
0	0.077	0.075	0.076	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	97.700	243.620	800.790	89.650	221.030	745.990
Standard deviation	5.240	10.010	42.840	7.830	19.740	57.220
CV（%）	5.360	4.110	5.350	8.730	8.930	7.670

Recovery

The recovery of Mouse TFPI was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-104	97
EDTA plasma （n=8）	85-97	91
Cell culture media （n=8）	92-107	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Mouse TFPI and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-103	92-107	97-113
1:2	Average	94	98	103
1:4	Range	90-101	90-106	93-106
1:4	Average	96	97	100
1:8	Range	87-103	93-108	92-105
1:8	Average	94	99	99

For the recovery experiment, the samples were spiked with standard solutions of a specific concentration and then serially diluted to 2-fold, 4-fold, 8-fold, and 16-fold dilutions, respectively. The average recovery rate was subsequently calculated.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TFPI. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TFPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 13 nm. The OD value is proportional to the concentration of Mouse TFPI. You can calculate the concentration of Mouse TFPI in the samples by comparing the OD of the samples to the standard curve.

Background

Tissue Factor Pathway Inhibitor (TFPI), also called extrinsic pathway inhibitor (EPI) and lipoprotein-associated coagulation inhibitor (LACI), is a key Kunitz-type serine protease inhibitor regulating the extrinsic blood coagulation pathway. It directly inhibits factor X (Xa) and, in an Xa-dependent way, inhibits VIIa/tissue factor (TF) activity by forming a quaternary Xa/LACI/VIIa/TF complex, playing a vital antithrombotic role.TFPI is mainly produced by vascular endothelial cells; it is also expressed by vascular smooth muscle cells, megakaryocytes/platelets, monocytes and other cells, and detected in macrophages and T cells in atherosclerotic lesions. Its structure has three tandem Kunitz domains (K1, K2, K3) and a basic C-terminal region: K2 inhibits FXa’s active site, while K1 inhibits FVIIa’s to block the TF–FVIIa complex’s procoagulant activity.