Micro-Sample

Mini Sample Rat CXCL2 (Growth Regulated Oncogene Beta) ELISA Kit (E-MSEL-R0003)

Name: Mini Sample Rat CXCL2 (Growth Regulated Oncogene Beta) ELISA Kit
Brand: Elabscience
SKU: E-MSEL-R0003
Price: 150.0 USD
Availability: InStock

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Mini Sample Rat CXCL2 (Growth Regulated Oncogene Beta) ELISA Kit - 1

Size	Price	Qty
96T	$ 510.00	- +
48T	$ 408.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

The sample size of MS series ELISA kit is only 1/4 of that of the traditional sandwich ELISA kit, which can effectively solve the problem of insufficient sample size.

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	25 μL
Manual Operation Time	1 h
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes CXCL2 in samples.No significant cross-reactivity or interference between CXCL2 and analogues was observed.
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.345	2.385	2.365	2.293
500	1.539	1.591	1.565	1.493
250	0.837	0.873	0.855	0.783
125	0.478	0.498	0.488	0.416
62.5	0.278	0.260	0.269	0.197
31.25	0.161	0.165	0.163	0.091
15.63	0.121	0.125	0.123	0.051
0	0.072	0.072	0.072	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	50.940	94.870	361.580	51.110	102.730	355.880
Standard deviation	2.420	4.030	20.280	3.880	6.450	23.380
CV（%）	4.750	4.250	5.610	7.590	6.280	6.570

Recovery

The recovery of Rat CXCL2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-106	100
EDTA plasma （n=8）	92-106	99
Cell culture media （n=8）	94-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CXCL2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-96	89-100	83-97
1:2	Average	91	94	90
1:4	Range	87-101	88-101	85-99
1:4	Average	92	95	90
1:8	Range	82-96	87-97	81-92
1:8	Average	88	92	87

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	86.50-104.30
Sample 2（n=16）	86.57-109.57

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CXCL2 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CXCL2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CXCL2 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 52 nm. The OD value is proportional to the concentration of CXCL2 . You can calculate the concentration of CXCL2 in the samples by comparing the OD of the samples to the standard curve.

Background

CXCL2/GRO beta, also called MIP-2 in mouse and CINC-3 in rat, is a member of the CXC chemokine family. Human CXCL2/GRO beta is 107 amino acids (aa) in length with a predicted molecular weight of 11 kDa. The mouse and rat orthologs share 70% and 71% aa sequence identity with the human protein, respectively. N-terminal aa 1-4 of CXCL2/GRO beta can be post-translationally cleaved which confers enhanced hematopoietic bioactivity. CXCL2/GRO beta is produced by a variety of cell types including monocytes and macrophages at sites of inflammation and is chemotactic for granulocytes, including neutrophils.