Mouse ACP3/PAP (Prostatic Acid Phosphatase) ELISA Kit (E-EL-M3110)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to ACP3 . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ACP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ACP3 , biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of ACP3 . You can calculate the concentration of ACP3 in the samples by comparing the OD of the samples to the standard curve.

ACPP,also known as prostatic acid phosphatase,belongs to the histidine acid phosphatase family. It has phosphotyrosyl phosphatase activity. It is predominantly expressed in the prostate gland,and restricted to gladular and ductal epithelial cells,and mainly in the secreted form. It is also widely expressed in non-prostatic tissues,such as bladder,kidney,pancreas,lung,cervix,testis,and ovary,as a type I transmembrane protein with N-terminal phosphatase activity. The expression of ACPP correlates with prostate cell proliferation. High levels of ACPP leads to slow cell growth. Though the level of ACPP in the circulation is increased in prostate cancer patients,its level and activity are reduced in prostate cancer cells. ACPP serves as a tumor suppressor. Reduced ACPP levels result in hyperphosphorylation of HER-2 (human epidermal growth factor receptor-2),activating downstream MAPK signaling,prostate cancer progression and androgen-independent growth. In addition,ACPP dephosphorylates ErbB-2,blocking downstream ERK1/2 and AKT signaling and tumorigenicity.