Mouse C-P(C-Peptide) ELISA Kit (E-EL-M0354)

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse C-P. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse C-P and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse C-P, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse C-P. You can calculate the concentration of Mouse C-P in the samples by comparing the OD of the samples to the standard curve.

C-peptide (connecting peptide, C-P) is a 31-amino-acid peptide that links the A and B chains within proinsulin and is an intrinsic component of insulin biosynthesis. It is generated in pancreatic beta cells and cleaved from proinsulin by prohormone convertases within secretory granules, then released into the circulation in equimolar amounts with insulin. Because it directly reflects beta-cell secretory activity, C-peptide provides an index of endogenous insulin output. C-peptide possesses anti-inflammatory, anti-apoptotic and microvascular-protective activities, improving endothelial function and tissue perfusion. Unlike insulin, it undergoes little hepatic first-pass clearance and has a longer half-life, and it is eliminated mainly by renal filtration, so it can also be measured in urine. Its slow clearance and equimolar secretion with insulin make it a stable and direct marker of residual beta-cell function. Measurement of C-peptide is used to distinguish type 1 from type 2 diabetes, to assess residual beta-cell function in insulin-treated patients, to investigate the causes of hypoglycemia and to monitor graft status after islet transplantation. This ELISA kit enables sensitive and specific quantitative determination of C-peptide in serum, plasma and urine, serving diabetes research.